KeyTec® Luminescent 3D Cell Viability Detection Kit

Luminescent Assay for Detecting the Number of Viable Cells in 3D cells culture
•  Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process
•  High Sensitivity: Stronger lysis ability, Wider linear range with low background and high signal
•  The luminescence signal is not only high but also robust, with a half-life of approximately 3-5 h. It is suitable for most assay formats

Size: 10 mL2*50 mL10*50 mL2*250 mL

Price: $ 144

CAT.: A2010201N

Delivery: Available in Stock

KeyTec® Luminescent 3D Cell Viability Detection Kit

Luminescent Assay for Detecting the Number of Viable Cells in 3D cells culture

• Easy to Use: A homogeneous assay - just follow the 'add-incubate-measure' process • High Sensitivity: Stronger lysis ability, Wider linear range with low background and high signal • The luminescence signal is not only high but also robust, with a half-life of approximately 3-5 h. It is suitable for most assay formats

Size: 10 mL2*50 mL10*50 mL2*250 mL

CAT.: A2010201N

Delivery: Available in Stock

Price: $ 144

Overview

KeyTec® Luminescent 3D Cell Viability Detection kit (3D LCV) is designed for 3D cell viability detection. It quantifies viable cells by measuring the ATP present, a key indicator of metabolically active cells. The homogeneous assay procedure involves adding a single reagent directly to 3D cells cultured in serum-supplemented medium. 
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin and luciferase react with ATP released by 3D cells, generating a sensitive and robust luminescent signal. With a luminescent signal half-life of 3-5 hours, this kit ensures high sensitivity and robust performance, making it an ideal choice for high-throughput screening (HTS), 3D cell proliferation and cytotoxicity assays.

Performance

Use KeyTec® Luminescent 3D Cell Viability Detection kit to detect 3D cell viability of Hela cell line. The results showed a linear relationship between the luminescent signal and the number of cells (5 - 200,000 cells/well). Hela cells, cultured in RPMI 1640 medium with 10% FBS, were serially diluted two-fold, starting from 200,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in Section 5.“Assay Procedure”. Ten minutes after adding the reagent, measure the luminescent signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Protocols

Protocol_A2010201N ∕ A2010202N ∕ A2010203N ∕ A2010204N _KeyTec® Luminescent 3D Cell Viability Detection Kit_VKEYBIO-02-2024.pdf

Components

CAT.

Description

Size

A2010201N

KeyTec® Luminescent 3D Cell Viability Detection Kit

10 mL

Each kit contains sufficient reagents to perform 100 tests of 100 μL/well.  
The kit contains the following components:      
       •  1 × 10 mL KeyTec® Luminescent 3D Cell Viability Detection Reagent

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-40 ℃

Related Products

Overview

KeyTec® Luminescent 3D Cell Viability Detection kit (3D LCV) is designed for 3D cell viability detection. It quantifies viable cells by measuring the ATP present, a key indicator of metabolically active cells. The homogeneous assay procedure involves adding a single reagent directly to 3D cells cultured in serum-supplemented medium. 
The detection principle is based on Luminescent technology. Within the kit, D-Luciferin and luciferase react with ATP released by 3D cells, generating a sensitive and robust luminescent signal. With a luminescent signal half-life of 3-5 hours, this kit ensures high sensitivity and robust performance, making it an ideal choice for high-throughput screening (HTS), 3D cell proliferation and cytotoxicity assays.

Performance

Use KeyTec® Luminescent 3D Cell Viability Detection kit to detect 3D cell viability of Hela cell line. The results showed a linear relationship between the luminescent signal and the number of cells (5 - 200,000 cells/well). Hela cells, cultured in RPMI 1640 medium with 10% FBS, were serially diluted two-fold, starting from 200,000 cells per well in a 96-well plate. Perform the assay according to the procedure outlined in Section 5.“Assay Procedure”. Ten minutes after adding the reagent, measure the luminescent signal using the Envision's Luminescence program. (Example program details include Mirror: Luminescence, Em filter: Luminescence 700, Measurement height: 6.5 mm, and Measurement time: 1 s).

Protocols

Protocol_A2010201N ∕ A2010202N ∕ A2010203N ∕ A2010204N _KeyTec® Luminescent 3D Cell Viability Detection Kit_VKEYBIO-02-2024.pdf

Components

CAT.

Description

Size

A2010201N

KeyTec® Luminescent 3D Cell Viability Detection Kit

10 mL

Each kit contains sufficient reagents to perform 100 tests of 100 μL/well.  
The kit contains the following components:      
       •  1 × 10 mL KeyTec® Luminescent 3D Cell Viability Detection Reagent

Notices

Certificate of Analysis

Limitations

Storage Conditions

For research use only

-40 ℃